Although Cas12f proteins benefit from compact size for delivery, their gene-editing activity remains limited. By adding an N-terminal α-helix to Cas12f, we created hpCasMINI, hpOsCas12f1, and hpAsCas12f1 variants that show improved gene activation and DNA cleavage in mammalian cells and mice.
- Shufeng Ma
- Kaitong Liao
- Zhili Rong
