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FFPE tissues are widely used to preserve clinical specimens, but formalin-induced crosslinking limits their use in epigenomic profiling. Here, the authors present spatial FFPE-ATACseq, enabling in situ chromatin accessibility mapping while preserving tissue architecture in archived samples.

A stereotaxic atlas of the whole mouse brain, based on a Nissl-stained cytoarchitecture dataset with isotropic 1-μm resolution, achieved through continuous micro-optical sectioning tomography, promises to be a versatile brainsmatics tool for studying the whole brain at single-cell level.

Chen, Zhou, Xue et al. show that psychological stress leads to noradrenaline release and Alkbh5 downregulation, causing the transfer of extracellular vesicles containing m⁶A-modified RNAs to neurons to regulate innervation and pancreatic cancer development.

The authors utilize the pulsed electric stimulus near the critical electric field to perform the antiferroelectric-ferroelectric phase transition engineering, realizing enhancement and quick stabilization of capacitance in antiferroelectrics.

The authors demonstrate a chemopiezoelectric effect in which the displacive migration of oxygen vacancies driven by an electric field induces a large strain in the surface layer of thin (K,Na)NbO₃ ceramics. They achieve an electrostrain of 1.9% under a field of −3 kV mm⁻¹, with thermal stability up to 200 °C.

Extensive intraoperative peritoneal lavage (EIPL) has been proposed as an approach to reduce peritoneal metastasis in patients with locally advanced gastric cancer undergoing gastrectomy. Here the authors report the results of the multicentric phase III SEIPLUS trial, showing that EIPL does not improve 3-year survival rate in patients with advanced gastric cancer.

The development of three-dimensional (3D) covalent organic frameworks (COFs) with novel topologies is of both fundamental and practical interest but the construction of highly crystalline 3D COF remains challenging. Here, the authors report highly crystalline 3D COFs with pto and mhq-z topologies by rationally selecting rectangular-planar and trigonal-planar building blocks with appropriate conformational strains.

Zhong et al. show that, in response to chimeric antigen receptor (CAR) T cell targeting, solid tumors increase the secretion of small extracellular vesicles carrying tumor antigens that are transferred to CAR T cells, resulting in fratricide and reduced antitumor activity.

Late Cretaceous left-lateral strike-slip faulting indicates a major change in the subduction direction of the Paleo-Pacific Plate beneath Eurasia, according to a method that uses zircon (U-Th)/He thermochronological data to detect strike-slip faults.

HIV-1 replication in resting CD4+ T cells is inefficient due to host restriction factors. Here, the authors show that KRT72 is a Vpx-counteracted antiviral factor that binds incoming capsids to the cytoplasmic intermediate filament, thereby restricting HIV-1 infection in resting CD4+ T cells.

The electrochemical coupling of NO₃⁻ and CO₂ to produce urea using entangled iron porphyrins embedded in three-dimensional covalent organic frameworks is reported. The spatial localization of twin iron sites overcomes the unordered transfer of intermediates, enabling carbon–nitrogen coupling.

Low-loss superconducting aluminium cables and on-chip impedance transformers can be used to link qubit modules and create superconducting quantum computing networks with high-fidelity intermodule state transfer.

The molecular mechanisms underlying the late-onset adverse effects of radiotherapy remain to be explored. Here, the authors observe compromised wound healing capacity in irradiated skin from breast cancer survivors and highlight THBS1 as a key epigenetically primed wound repair-related gene.

The tumour microenvironment features influencing response to neoadjuvant immunochemotherapy (nICT) in esophageal squamous cell carcinoma (ESCC) remain to be explored. Here, single cell and TCR sequencing on pre- and post- nICT treatment ESCC tissues identifies the presence of CXCL13⁺CD8⁺ T cells as a predictor of improved response and the enrichment of TNFRSF4⁺CD4⁺ Tregs as a marker of treatment resistance.

A smartphone-based system, designed to induce a steady gaze in children using cartoon-like video stimuli, can identify visually impaired children across a wide range of ophthalmic disorders, based on analysis of gazing behaviors and facial features.

Here the authors provide safety and immunogenicity data for an AdV5-based SARS-CoV-2 vaccine, administered intramuscularly as heterologous booster after three-doses of inactivated SARS-CoV-2 vaccine in Chinese adults.

The mechanism driving inflammatory-to-proliferative state transition during wound healing remains unclear. Here, the authors discover that SNHG26 interacts with ILF2, redirecting it from inflammatory genomic loci to the LAMB3 locus, thereby promoting wound healing.

Mechanisms underlying tumor cell plasticity remain poorly understood. Here, the authors show the presence of a dormant-invasive SOX10- subpopulation in cutaneous melanoma that can be targeted by cIAP1/2 inhibitors.

Derivatives of fluorinated alkenes are commonly found in biologically active molecules, but their synthesis remains challenging. Towards this goal, a rhodium-catalysed method for the selective β- or γ-addition of nucleophiles to gem-difluoroallenes has now been developed. Catalysts with N- or P-based ligands result in the β- or γ-selective addition, respectively, of amines and thiols to gem-difluoroallenes.

DILITracer: an AI-driven ternary hepatotoxicity classification model via spatiotemporal analysis of human liver organoids brightfield images.

Non-classical states with a large, definite number of photons can now be produced in a superconducting cavity and used for quantum-enhanced sensing.

New treatment strategies are required for ulcerative colitis. Here the authors show in mouse models that cholesterol sulfate, an endogenous active cholesterol derivative, contributes to the healing of the mucosal barrier by promoting cholesterol biosynthesis in colonic epithelial cells and exhibits therapeutic efficacy against ulcerative colitis.

Centrosome duplication is synchronized with cell cycle events but how this is regulated at a molecular level is unclear. Here, the authors show that the DNA replication licensing factor Cdc6 restrains centrosome duplication via binding and inhibiting Sas-6, and the inhibition of Cdc6 on Sas-6 is negatively regulated by Plk4 phosphorylation.

A signalling mechanism ensuring intraspecies and interspecies reproductive barriers in flowering plants is uncovered.

NAD(P)H dehydrogenase-like complex NDH-1L couples the electron transport from ferredoxin (Fd) to plastoquinone (PQ) and proton pumping from cytoplasm to the lumen. Here authors report two structures of NDH-1L from Thermosynechococcus elongatus BP-1, in complex with one Fd and an endogenous PQ, respectively.

Hematite is a promising material for photoelectrochemical water splitting, but its mechanisms are not well understood. Here, the authors report a detailed mechanism, identifying key reaction intermediates and highlighting the influence of solvent water and oxygen desorption on the reaction pathways.

By map-based cloning and knockout experiments, a study identified and validated ZmDMP to be one of the two genes known to control haploid induction in maize. A single-nucleotide mutation in ZmDMP causes a 2–3-fold increase in the haploid induction rate.

Understanding the mutation landscape of cancer may enable the development of more targeted therapies. Here, the authors sequence a panel of genes in a large Asian cohort and compare to American cohorts and find 64% of the Asian patients have actionable mutations.

How nucleosome assembly of parental histones is regulated following DNA replication is still an open question. Here the authors show that unlike deposition of new histones H3.1 and H3.3 that utilizes different histone chaperones, parental H3.1 and H3.3 are both stably inherited during mitotic cell division in mouse embryonic stem cells, and this involves histone chaperones Mcm2, Pole3 and Pole4.

The authors report on the emergence of intercell moiré exciton complexes where, in H-stacked WS₂/WSe₂ heterobilayers, the exciton’s hole from the WSe₂ layer is surrounded by its bound electron’s wavefunction distributed among three adjacent moiré traps in the WS₂ layer exhibiting an out-of-plane dipole and in-plane quadrupole.

Here, the authors revealed molecular heterogeneity, developmental trajectory and regulatory network of early human pancreas development, and depict the whole progression of pancreatic organogenesis during the first trimester at the single-cell level.

Here the authors propose a mechanism for direct space-time manipulation of ultrafast light field and experimentally demonstrate the generation of a spatiotemporally coupling light spring with a broad topological charge bandwidth.

Here, the authors report large photothermal nonlinearity mediated by anapole states within subwavelength Si nanodisks, offering a mechanism for dynamic tuning of far-field radiation from multipolar modes. Nonlinear scattering is used to demonstrate the potential for far-field optical localization of Si nanostructures.

A small cluster of brainstem-projecting layer 5 neurons in primary motor cortex elicit contraction of the bladder muscle and trigger urination. These findings open new directions for treating urination-related disorders.

Heterogeneity in gene expression and epigenetic states exists across individual cells. Here, the authors develop scCAT-seq, a technique for simultaneously performing ATAC-seq and RNA-seq within the same single cell.

Few-nanometre-thick flakes of trigonal and monoclinic Cr₅Te₈ can be grown using chemical vapour deposition, with the monoclinic phase exhibiting an anomalous Hall conductivity of 650 Ω^–1 cm^–1 and anomalous Hall angle of 5%.