Fig. 5: USP24 inhibits the proliferation and migration of HCC cells. | Communications Biology

Fig. 5: USP24 inhibits the proliferation and migration of HCC cells.

From: USP24 promotes autophagy-dependent ferroptosis in hepatocellular carcinoma by reducing the K48-linked ubiquitination of Beclin1

Fig. 5

A, B The overexpression efficiency of USP24 by lentivirus was measured by western blot and qRT-PCR (n = 3). C CCK-8 assays were conducted to determine the viability of SMMC-7721 and HCCLM3 cells after overexpressing USP24 (n = 3). D–E Colony formation assays of HCC cells treated with LV-CON or LV-USP24 were performed to evaluate the ability of proliferation. The clone number was counted (n = 3). F Migration abilities of SMMC-7721 and HCCLM3 cells after overexpression of USP24 were evaluated by wound healing assay. Scale bar, 1 mm. G-H Transwell assays were performed to detect the ability of migration, and the migration rate was calculated (n = 3). Scale bar, 100 μm. **P < 0.01, ***P < 0.001, ****P < 0.0001.

Back to article page