Fig. 8: USP24 inhibits HCC growth in vivo.

A The process of constructing subcutaneous tumor models in nude mice. B–C Image of subcutaneous tumors in control and USP24 overexpressed groups (n = 5). D The tumor weight is presented as the mean ± standard deviation (n = 5). E Tumor volumes were calculated every 3 days after injection. F The body weight of nude mice during this experiment (n = 5). G Representative images of H&E stained tumor tissues. H Representative images of IHC stained USP24 and Ki-67 in control tissues or USP24 overexpressed xenograft tumor tissues. I The quantification of USP24 and Ki-67 positive cells (n = 5). J Western blot was performed to detect the expression of proteins associated with autophagy and ferroptosis. K Graphical summary of USP24 regulated autophagy-dependent ferroptosis in HCC. ****P < 0.0001.