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Beta-hydride elimination is a crucial elementary step in transition-metal catalysis, but controlling the stereochemistry of this process has been underdeveloped. Here, the authors report a Trost-ligand-enabled enantioselective desymmetric beta-hydride elimination reaction from π-allyl-palladium species, providing rapid access to cyclohexenes bearing a remote C4 stereocenter.

Efficient delivery systems are urgently needed for genome editing in monocot plants. Here, this study develops a system of virus-induced genome editing in tillers (ViGET) to achieve heritable editing in wheat bypassing transgene and tissue culture.

The dissatisfied delivery efficiency of drugs to lungs and other metastatic sites limit the corresponding therapeutic efficacy. Here this group reports an inhalable surfactant-based nanoliposome system to co-deliver Osimertinib and DNA plasmid which is capable of fusing into pulmonary surfactant, penetrating through physiological barriers and targeted accumulating in lung tissues.

Polyamide membranes with ultra-high negative charge density demonstrate exceptional removal of divalent ions and organic pollutants without compromising water permeance.

Challenges of zinc electrodes imped their progress in energy storage. Here, authors propose a parts-per-million scale electrolyte additive, phosphonoglycolic acid, enabling Zn stripping/stripping for nearly a year in coin cells, and exhibiting high durability in pouch cells and redox flow batteries.

Electrochemical conversion of nitrate into ammonia offers a method for mitigating nitrate pollution in water. Here the authors report the electrochemical nitrate-to-ammonia conversion using high-entropy oxide Mg_0.2Co_0.2Ni_0.2Cu_0.2Zn_0.2O and find that the Co spin states are crucial for synergistic ammonia generation.

Heterobilayers of transition metal dichalcogenides host moiré superlattices that give rise to strong electron interactions. Here, the authors study the photoluminescence from interlayer excitons in a WS₂/WSe₂ heterobilayer to reveal the onset of various correlated insulating states.

In this study, the authors isolate and characterize BBIBPCorV inactivated vaccine-elicited human antibodies. They show that these can broadly neutralize a range of SARS-CoV-2 variants and protect mice from Delta and Omicron infection. The neutralization mechanism of bispecific antibodies were solved structurally.

In situ profiling of lysosomal proteomes is impeded by the acidic and digestive environment of lysosomes. Now, a bioorthogonal photocatalytic system (CAT-Lyso) is developed that is compatible with these conditions, enabling the proximity labelling of lysosomal proteomes within living cells.

Wang et al. develop data-independent acquisition activity-based protein profiling, a quantitative chemoproteomic method to globally profile protein dopamination in the mouse brain, and identify a protective role of dopamine in regulating the function of microtubule-associated protein Tau.

The functions of non-coding regulatory elements can be systematically studied genome-wide at high throughput in human cells via their Cas9-mediated deletion through libraries of paired single-guide RNAs targeting both ends of each element.

How RNA polymerase II subunits enter the nucleus is not well understood. Here, the authors show that Transport and Golgi organization protein 6, TANGO6, recruits RNA polymerase II subunit B2, RPB2, to the ER membrane in a retrograde manner and transports it to the nucleus with the aid of importins.

Chemoresistance limits the success of chemotherapy in patients with cancer. Here, the authors perform 30 genome wide CRISPR knockout screens to identify genes associated to resistance against commonly used chemotherapeutics across multiple cancer types, followed by 26 second round CRISPR screens to identify druggable targets of chemoresistance.

Modulating amyloid protein phase separation and fibrilization may help in addressing neurodegenerative diseases. This study demonstrates that halogen-doped graphene quantum dots can modulate these processes in TDP-43 in both nucleus and cytoplasm.

Aberrant circRNAs expression in repair cells is intricately linked to deficits in wound healing. Here, the authors engineered extracellular vesicles enriched with circCDK13 and demonstrate their ability to facilitate diabetic wound repair.

Tamlo-R32, an engineered wheat mutant allele of the Mildew resistance locus O (MLO) gene, confers resistance to powdery mildew, retains robust wheat growth, and can be transferred to other agriculturally important wheat varieties.

Determining glioma types directly from whole-slide images (WSIs) would be of great diagnostic utility. Here, the authors develop a deep learning model to identify diffuse glioma types from WSIs according to the 2021 WHO classification across multiple cohorts and with interpretable features.

Technical advancements have significantly improved early diagnosis of cervical cancer, but accurate diagnosis is still difficult due to various practical factors. Here, the authors develop an artificial intelligence assistive diagnostic solution to improve cervical liquid-based thin-layer cell smear diagnosis according to clinical TBS criteria in a large multicenter study.

A λ exonuclease system exhibits protospacer-adjacent motif-independent DNA-binding specificity, enabling diagnostic and imaging applications.

The chaperone Hsp27 prevents FUS from undergoing liquid–liquid phase separation until stress-induced phosphorylation causes Hsp27 to partition with FUS to preserve the liquid phase against amyloid fibril formation.

Pathologic α-synuclein spreads from cell-to-cell through binding to the lymphocyteactivation gene 3 (Lag3). Here, the authors demonstrate that the amyloid β precursor-like protein 1 (Aplp1) interacts with Lag3 and facilitates the binding, internalization, transmission, and toxicity of pathologic α-synuclein.

Cas13 can intrinsically target host RNA in mammalian cells through previously unrecognized mechanisms without the involvement of a CRISPR RNA, and host genes involved in viral processes can constrain the lentiviral delivery and expression of Cas13.

Dalpiciclib is a CDK4/6 inhibitor, recently approved for treatment in advanced breast cancer patients in China. Here, the authors report the results of a phase II trial investigating oral dalpiciclib (CDK4/6 inhibitor) and pyrotinib (pan-HER inhibitor) in patients with HER2-positive metastatic breast cancer.

Here, the authors report the discovery of a redox switch for AcrIIC1, which regulates AcrIIC1’s monomer-dimer interconversion and inhibitory activity on Cas9. By reporting the structures of different redox states of NmeAcrIIC1, they identify a pair of conserved cysteines which mediate the redox switch. The replacement of the redox-sensitive cysteines allows the generation of a more stable and robust variant under various cellular environments.

An out-of-plane magnetic field is expected to strongly modify exciton-phonon interactions in atomically thin transitional metal dichalcogenides. Here, the authors show that the phonon-exciton interaction in monolayer WSe₂ lifts the inter-Landau-level transition selection rules for dark trions.

Thrombotic cerebro-cardiovascular diseases are the leading causes of disability and death worldwide but current drug therapeutics show important limitations. Here, the authors exploit a selfpropelling nano-penetrator with high fuel loading and controllable motion which is molecularly co-assembled using a photothermal photosensitizer and a photothermal-activable NO donor.

Here, the authors use cryo-EM to show that phosphorylating or O-GlcNAcylating α-synuclein on serine 87 leads to the formation of two distinct fibril structures. Both structures display reduced neurotoxicity and propagation activity.

Real-time continuous sensing of biological analytes is of importance in a range of biomedical applications. Here, the authors report on a flexible and physically transient sensor for the detection of nitric oxide and demonstrate applications in nitric oxide sensing in organs ex vivo and in vivo.

Thyroid hormone (TH) action regulates brown adipose tissue thermogenic capacity through incompletely understood mechanisms. Here the authors report that T3, the active form of TH, increases thermogenic capacity via thyroid hormone receptor α-mediated hyperplasia of brown adipose tissue adipocyte progenitor cells.

Current techniques for visualizing cell generated forces suffer from throughput limitations. Here, Gu et al. introduced photonic crystal cellular force microscopy, inspired by chameleons, enabling visualization and quantification of vertically directed cell forces, well-suited for drug screening.

Application of CRISPR-Cas13d is limited by the inability to predict on- and off-targets. Here the authors perform CRISPR-Cas13d proliferation screens followed by modeling of Cas13d on- and off-targets; they design a deep learning model, DeepCas13, to predict the on-target activity of a gRNA.

Increasing studies have suggested that single reference genome is insufficient to capture all variations in the genome. Here, the authors report a graph-based cucumber pan-genome by analyzing 12 chromosome-scale assemblies and reveal variations associated with agronomic traits and domestication.

Charge-to-spin conversion allows for the generation and control of spin polarization via a charge current. Typically, this is done with non-magnetic materials with large spin-orbit interactions such as Platinum. Herein, Dai et al demonstrate an intriguing charge-to-spin mechanism, a magnetic spin Hall effect, in a van der Waals heterostructure.

Staphylococcus capitis is a common causative agent of bloodstream infections in neonatal intensive care units, with multidrug resistant isolates complicating treatment. Authors aimed to establish a core genome multilocus sequence typing (cgMLST) scheme to document the transmission and dissemination of multidrug-resistant S. capitis isolates.

Voltage-gated sodium channels function as multiprotein signaling complexes. Here, authors show that the dispanin TMEM233 is essential for activity of stinging nettle toxins and that co-expression of TMEM233 modulates the gating properties of Na_V1.7.

EB are conserved microtubule (MT) plus-end binding proteins. Here, Yang et al. report that Plasmodium EB1 possesses distinct MT-lattice affinity and decorates the full-length of spindle MTs. Gene deletion impairs the spindle-kinetochore lateral attachment, leading to anucleated male gametes.

Vesicle associated membrane protein 2 (VAMP2) contains a conserved SNARE motif that interacts with syntaxin-1 and SNAP25 for SNARE complex assembly. Here authors use in-cell NMR spectroscopy to describe the dynamic membrane association of VAMP2 SNARE motif in mammalian cells at the atomic resolution.