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The possibility to trap biomolecules is important for analysing them by optical methods. Here we show how nanoscale chambers with macromolecular gates can be used to trap hundreds of proteins in a volume of one attoliter at physiological conditions without exposing them to any direct forces.

Mesocosm experiments revealed that both phytoplankton community composition and cellular acclimation influence marine particulate C:N:P ratios, with community shifts more sensitive to nitrogen supply and acclimation to the nutrient N:P supply ratio

Cell migration in confined environments is initiated by a cytoplasmic pool of anillin and Ect2 that promotes RhoA/myosin II-mediated activation at the poles of migrating cells, in a process dependent on the extracellular environment stiffness.

This protocol describes procedures for speed-breeding approaches using growth cabinets and LED-supplemented glasshouses. The approaches can be used to accelerate crop research and are compatible with a wide variety of crops.

Dietary n-6 PUFAs enhance adipose tissue expandability through the PPARγ–LPCAT3 membrane remodelling axis.

This study demonstrates that growth arrest under stress in Arabidopsis protects meristem cells from DNA damage, challenging the idea that it is merely due to energy trade-offs and highlighting its role as an active defense strategy.

Neuropixels 1.0 NHP is a 45-mm, high-density silicon probe capable of recording large numbers of neurons with single-neuron resolution from most areas in a macaque’s brain.

Many montane birds seasonally migrate between elevations. This study shows two bird species exhibit phenotypic plasticity in response to this altitude shift: the small-scale elevational migrant shows greater temperature-driven plasticity, while the large-scale migrant displays stronger hypoxia-driven plasticity.

Hyperpolarized ¹²⁹Xe gas is used as a contrast agent in nuclear magnetic resonance imaging. Here, the authors demonstrate a microfluidic chip that optically polarizes ¹²⁹Xe gas for compact nuclear magnetic resonance imaging applications.

A semiconductor chip, coupled to an ion-sensitive field effect transistor (ISFET) pH sensor, can amplify and quantitate DNA in real time without dyes, cameras and external heating devices.

Dynamic cytoskeletal regulation of lymphatic endothelial cell shape, induced by isotropic stretch and crucial for dermal lymphatic capillary function, is identified and found to result from continuous remodelling of cellular overlaps that maintain vessel integrity.

A single-shot full-vector-field measurement technique for intense, ultrashort laser pulses is studied, demonstrating the approach on systems ranging from high-repetition-rate oscillators to petawatt-class lasers.

The degree to which the diurnal light/dark cycle influences Mg-heterogeneity in foraminifera with complex shell morphologies remains unknown. Here, using highly spatially resolved analytical techniques, the authors investigate Mg-banding and calcification in isotope-labelledNeogloboquadrina dutertreispecimens.

Human airway contains physiologically relevant yet rare cells, but their scarcity prevents thorough profiling and differentiation studies. Here the authors use single cell RNA sequencing to identify rare ionocytes and tuft cells, as well as a potential progenitor population with cytokine-guided differentiation into either the ionocytes or tuft cell lineage.

Solid-state pressure sensors have performance limitations in liquid environments. Here, the authors design a pressure sensor using solid–liquid–liquid–gas multiphasic interfaces where a trapped air layer modulates capacitance changes with pressure to achieve near-friction-free contact line motions for near-ideal pressure sensing.

By analyzing bulk and single-cell RNA-sequencing datasets generated from left atrial appendages of individuals with atrial fibrillation and non-affected controls, Leblanc and Yiu identify cell-type-specific genes and transcriptomic programs implicated in atrial fibrillation, a cardiomyocyte-specific androgen receptor signaling signature and an anti-fibrotic effect of NR4A1 inhibition in atrial cardiofibroblasts.

Rhine et al. find that neuronal aging leads to widespread dysregulation of RNA biology, including mislocalization of splicing proteins like TDP-43, chronic cellular stress and reduced resiliency.

Scale models of the human left ventricle made of tissue-engineered nanofibrous scaffolds and primary rat cardiomyocytes or human-stem-cell-derived cardiomyocytes enable the study of contractile function and the modelling of structural arrhythmia.

Dual leucine-zipper kinase (DLK) drives several forms of neurodegeneration but inhibitors of DLK’s kinase ___domain cause adverse effects. Zhang et al describe an alternative strategy to block DLK signaling by targeting the axonal palmitoylation of DLK.

Sepsis may promptly develop into lethal organ failure, so early diagnosis and treatment planning are essential. Here the authors use machine learning to develop a six-gene signature, termed Sepset, for initial diagnosis, and integrate Sepset into a microfluidic-based bench-side platform for predicting the prognosis of suspected sepsis suitable for the clinic.

Approach versus avoidance responses are driven by D1 and D2 neurons in the ventral hippocampus.

Thermogenic proteins like PGC1α are downregulated under thermal stress, but the underlying mechanisms are unclear. Here, the authors show that chaperone-mediated autophagy, regulated by PARK7, controls PGC1α stability and BAT function at thermoneutrality.

Here the authors show that codependence of dynein and kinesin KIF1C occurs through binding of the FTS–HOOK3–FHIP1B cargo adapter. Binding of KIF1C releases the HOOK3 autoinhibited folded conformation allowing dynein to bind the adapter. In this cocomplex, KIF1C further acts as a processivity factor for dynein.

Sadhu et al. identify and functionally validate ferredoxin-1 (FDX1) as a determinant of cholesterol metabolism and cardiovascular risk in Asian populations.

The functions of small nucleolar RNAs (snoRNAs) in cancer remain to be studied. Here the authors show that the snoRNA Snord67 promotes lymph node metastasis of breast cancer cells through guiding modifications of the spliceosome and regulating the splicing landscape.

Tummino et al. dock 74 million molecules against the human cannabinoid-1 receptor to find uM ligands. Optimization led to a nM agonist conferring analgesia with reduced side effects in mice, highlighting its potential as a pain therapeutic and the promise of a structure-based approach.

Inhibition of haem synthesis is shown to lead to the accumulation of branched-chain amino acids in the brown adipose tissue of mice, which reduces UCP1 levels and impairs thermogenesis.

Neuron-specific DNA methylation and intragenic enhancers target the Rett syndrome protein methyl-CpG-binding protein 2 to regulate genes that delineate closely related neuron types, suggesting breakdown of fine-scale neuronal specification in developmental disorders.

The taurine–taurine transporter axis is a critical dependency of aggressive myeloid leukaemias.

Zhang et al. show that dynamin forms a helical structure with actin and, upon disruption, enhances branched actin polymerization, constituting a dynamic cycle to regulate actin cytoskeleton mechanical strength.

Zhu et al show that LRRK2 kinase promotes invagination of the plasma membrane to create ‘reservoirs’, which dynamically store or release excess membrane. Salmonella can exploit these membrane reservoirs during invasion of host cells by controlling the activity of LRRK2.

Establishing pH differences in aqueous flow batteries widens their voltage window, but acid–base mixing shortens their lifespan. In this study, the authors introduced a pH recovery system to address crossover issues, ensuring long-lasting, high-voltage pH-decoupled flow batteries.

Optogenetically induced chemo-mechanical excitations are used to drive and study shape deformations in starfish oocytes. Understanding and eventually controlling such waves is important for the development of synthetic cells.

Here the authors reveal triple heterogeneity of H2A.Z containing nucleosomes, attributed to interactions involving the C-terminal tail of the histone variant, and demonstrate modulation of global chromatin architecture using a synthetic tail peptide.

Xylem-mediated transport and light intensity on leaf surface primarily drive foliar methane oxidation and nitrous oxide emissions in Salix bebbiana plant, according to an analysis of an ecophysiological manipulation experiment with biochar incorporation and nitrogen addition.

The Marius Hills, located in central Oceanus Procellarum, form the largest volcanic dome complex on the Moon. Here, gravity data is used to image the magmatic structures in this region. Magmatic conduits connect the northern and southern intrusions of Marius Hills and link them with the structures along Procellarum’s western border.

The gut hormone tachykinin (Tk) is found to be regulated by protein intake in flies and mice. In flies, Tk is shown to impact food choices, sleep and lifespan.

Methane emissions from aquatic systems contribute approximately half of global methane emissions, according to meta-analysis of natural, impacted and human-made aquatic ecosystems and indicating potential mitigation strategies to reduce emissions.

Multimodal results (iEEG, fMRI and MEG) of predictions from integrated information theory and global neuronal workspace theory align with some predictions of both theories on visual consciousness, but also critically challenge key tenets of both theories.

Deploying two unrelated CRISPR nucleases in tandem, with multiplexed CRISPR RNAs and a chemically stabilized activator, creates a simple, one-step assay that can rapidly detect attomolar concentrations of RNA without needing target amplification.

Ibogaine is a natural substance that interrupts opioid addiction but has cardiac risks. This article introduces novel ibogaine analogs that show reduced cardiac risk and enhanced neuroplasticity and therapeutic-like effects in models of opioid use disorder.